A novel strategy for Escherichia coli detection in raw beef in combination with click chemistry
Year: 2025
Type: article
Abstract: Bacteria-assisted click chemistry for enhanced nanozyme-based Escherichia coli ( E. coli ) detection. The size and shape of the alkyne-modified Fe 3 O 4 @Au NPs were 110 nm and urchin-like, respectively. The azide-functionalized Au QDs were spherical in shape, and their size was ~5 nm. The absorbance and emission peaks of Fe 3 O 4 @Au NPs and Au QDs were located at 526 nm and 520 nm, respectively. The nanozymatic activity of both materials significantly improved in the presence of Cu 2+ , E . coli, and 3,3′,5,5′-tetramethylbenzidine (TMBZ)/H 2 O 2 mixture. Here, the bacterial metabolic product Cu + was utilized to trigger the click reaction between alkyne-modified Fe 3 O 4 @Au NPs and azide-functionalized Au QDs which enables magnetic separation. The calculated limits of detection (LODs) of E. coli were as high as 5 CFU/mL and 25 CFU/mL in the PBS buffer and spiked raw meat samples, respectively. The proposed nanozymatic E. coli assay was selective in the presence of other bacteria.
Source: npj Science of Food
Author: Abdulhakeem Alzahrani
Institution: King Saud University
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,790
Funder: King Saud University
Award: G736424374
OpenAlex ID: W4409767598