Effect of extraction solvents on total polyphenolic content, total flavonoid content, and antioxidant activity of Tunisian cultivated mulberry (Morus alba L.) fruit extracts
Year: 2025
Type: article
Abstract: This study aims to evaluate the total polyphenolic, flavonoid contents, and the antioxidant activity of the aqueous, ethanolic, and methanolic extracts of Tunisian cultivated mulberry fruits.Analyses showed that the highest extract yield was obtained in the methanolic extracts 44.15%.The highest values of total phenolic content (TPC) were obtained in aqueous extract (6.75 ± 0.38 mg gallic acid equivalent per gram dry extract, mg GAE/g DE) followed by the methanolic extracts (6.10 ± 0.23 mg GAE/g DE), and the ethanolic extract (4.98 ± 0.55 mg GAE/g DE).Similarly, the total flavonoid content (TFC) was also detected in a significant amount with values ranging from 2.79 ± 0.21 mg quercetin equivalent per gram dry extract (mg QE/g DE) in the ethanolic extract to 3.50 ± 0.18 mg QE/g DE in the methanolic extract.The antioxidant activity of different extracts was then assessed, and the IC50 reached the values of 0.83 ± 0.05, 1.13 ± 0.13, and 1.78 ± 0.14 mg/mL for the ethanolic, methanolic, and aqueous extracts, respectively.The results highlight the richness of white mulberry fruits in bioactive molecules and the effect of extraction solvents on the variation of antioxidant activity.Indeed, the antioxidant properties of white mulberry fruits may be promising for potential applications in the pharmaceutical and cosmetic industries.
Language: English
Subfield: Biochemistry
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4409780073