Frequency domain broadband short-wave infrared spectroscopy for measurement of tissue optical properties from 685 to 1300 nm
Year: 2025
Type: article
Abstract: SignificanceExtending frequency domain diffuse optical spectroscopy (FD-DOS) into the short-wave infrared (SWIR) region has the potential to improve measurements of key biological tissue chromophores such as water and lipids, given their higher absorption in SWIR compared with near-infrared wavelengths. Few studies have explored FD-DOS in the SWIR range.AimWe present the first demonstration of a frequency domain broadband SWIR spectroscopy (FD-Bb-SWIRS) system to measure optical properties from 685 to 1300 nm.ApproachA custom hybrid system was developed, combining discrete frequency domain measurements from 685 to 980 nm with broadband continuous wave measurements from 900 to 1300 nm. This setup provided absolute absorption (μa) spectra from 685 to 1300 nm. Validation was performed using mineral oil-based solid phantoms, deuterium oxide (D2O) liquid phantoms, and desiccating porcine tissue.ResultsThe FD-Bb-SWIRS system was sensitive to changes in μa from varying concentrations of absorbers in solid and liquid phantoms. Ex vivo measurements of μa spectra indicated differences in tissue water content across different porcine tissue samples during baseline and desiccation.ConclusionsFD-Bb-SWIRS is highly sensitive to μa in the 685 to 1300 nm range and enables precise quantification of water in biological tissues. It represents a significant step forward in advancing SWIR-based optical spectroscopy for clinical applications.
Source: Journal of Biomedical Optics
Authors: Diana Suciu, Thao Pham, Lina Lin Wei, Shripreetika Guruprasad, Darren Roblyer
Institution: Boston University
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,675
Award: G926489895
OpenAlex ID: W4409786872