Effects of two different peptides on pentylenetetrazole-induced seizures in larval zebrafish
Year: 2025
Type: article
Abstract: Epilepsy is a common and severe neurological disease characterized by spontaneous and recurrent seizures. Although anti-seizure treatments are effective for most patients, approximately 30% remain pharmacoresistant. Moreover, uncontrolled seizures are associated with increased health risks and shortened life expectancy in individuals with refractory epilepsy. Preclinical studies play a crucial role in drug discovery, and the zebrafish (Danio rerio) have been successfully employed for this purpose. In this study, we utilized the zebrafish PTZ-induced seizure model to evaluate the effects of two peptides on seizure responses: Tripeptide (p-BTX-I) and the CX2 (a Cx43derivated peptide). Zebrafish larvae at 6 days post-fertilization were pre-treated with these peptides at various concentrations, depending on their experimental groups, 24h prior to seizure induction. We assessed seizure frequency, quantified swimming activity, measured transcript levels of genes related to inflammation and apoptosis (il1b, tnfa, cox1, cox2a, il6, casp3a, casp9, baxa, bcl2a, and c-fos), and analyzed the biodistribution of both peptides. Our results indicate that the Tripeptide exhibited anti-inflammatory and anti-apoptotic effects, particularly through reducing the expression of il1b and casp9. CX2 pre-treatment significantly downregulated inflammatory markers (il1b, il6, tnfa, and cox1). Biodistribution analysis confirmed that the CX2 peptide reached the zebrafish brain, suggesting a direct role in modulating seizure-related pathways. Our findings demonstrate that Tripeptide and CX2 peptides can modulate gene expression and mitigate molecular response associated with epileptic seizures in the zebrafish brain. These peptides thus represent promising candidates for future research aimed at developing novel anti-epileptic therapies. However, additional studies are required to evaluate their long-term efficacy, elucidate underlying mechanisms of action, and explore potential translational applications.
Source: PLoS ONE
Institutions: Universidade Estadual de Campinas (UNICAMP), Brazilian Institute of Neuroscience and Neurotechnology, Universidade de Santiago de Compostela, Universidade da Coruña, Servicio Gallego de Salud, Instituto de Investigación Biomédica de A Coruña, Universidade de Vigo, Centro Singular de Investigación en Química Biológica y Materiales Moleculares
Language: English
Subfield: Psychiatry and Mental health
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,382
Awards: Functional studies of genes of inflammation and microRNAs related to seizures using the zebrafish model, G116629194, G1175115578, G1438505084, G1496055848, G210041235, G2233490251, G2262748287, G2567692413, G2601397997, G2823776494, G300979063, G34346518, G3480869486, G3681454997, G3694284866, G3876637791, G4042783231, G4057659932, G4092263876, G410571670, G425668148, G451917667, Twinning for Promoting Excellence, Ability and Knowledge to develop novel approaches for targeting inflammatory and degenerative age-related joint diseases, G5080475149, G5195188938, G5241663416, G5353516086, G5539308778, G5967599077, G67688110, G6818627905, G7084143925, G7434936867, G762602090, G7764612811, G8207841777, G929308097
OpenAlex ID: W4409798591