Work

Open-source software utilization for zebrafish embryos behavior test

Year: 2025

Type: article

Abstract: This work described simple methods for measuring locomotive activity using open-source software, ImageJ1.54fFiji, and VirtualDub2. The significance of animal behavior is a mirror of brain activity, which can give information implicated with neurological diseases. Commercial behavioral analysis software frequently needs expertise and expenses high costs due to equip a specific instrument to use of software, thereby encouraging a trend toward open-source alternatives that are both accessible and effective. Here, we explained how to convert video format, measure movement, and produce useful locomotive parameters to aid in the assessment of zebrafish embryos. This method could be easily translated for use in other model systems. This methodology seeks to streamline behavioral quantification in research contexts, encouraging broader research aspects.

Source: Molecules and Cells

DOI: 10.1016/j.mocell.2025.100221

Authors: Thilini Ranasinghe, Seon-Heui Cha

Institution: Hanseo University

Language: English

Topic: Zebrafish Biomedical Research Applications

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,500

Funders: National Research Foundation of Korea, Ministry of Science and ICT, South Korea