Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83
Year: 2025
Type: article
Abstract: Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is the member of starch degrading enzymes that catalyses an intramolecular transglycosylation reaction to produce cyclodextrins . CGTase producing 141 isolates were obtained from different agricultural fields (paddy, corn , potato, sorghum, and millet), gardens, and industrial waste soil samples. Amongst these 141, an alkaliphilic CGTase producing bacterial isolate Paenibacillus daejeonensis P-83 was selected. Although the CGTase showed the ability to produce α-, β-, and γ-cyclodextrins, the optimization studies were focused on β-CD production. One-factor-at-a-time (OFAT) increased the initial CGTase production from 1.05 U/ml to 2.76 U/ml with optimal conditions like, 3 % (w/v) water chestnut flour, 0.5 % (w/v) peptone , 0.5 % (w/v) yeast extract, 0.02 % (w/v) MgSO 4 , 0.1 % (w/v) K 2 HPO 4 , pH 11 ± 0.2, 6 % (v/v) inoculum, and incubation at 30 °C ± 0.2 for 72 hours on a rotary shaker (150 rpm). The CGTase production was increased upto 5.23 U/ml by statistical optimization via Central Component Design (CCD) using three variables i.e., water chestnut flour (3.65 %; w/v), peptone (1.1 %; w/v), and yeast extract (0.5 %; w/v). The optimization process significantly increased the CGTase production by 2.55 and 4.9 times using OFAT and CCD, respectively.
Source: The Microbe
Institution: Gujarat University
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,150
OpenAlex ID: W4409815935