Work

Characterization and Biotechnology of Three New Strains of Basidial Fungi as Promising Sources of Biologically Active Substances

Year: 2025

Type: article

Abstract: The study of new strains of basidiomycetes as sources of biologically active substances is a promising direction in modern biotechnology. This work aims to isolate new cultures of the fungi Daedaleopsis tricolor, Pycnoporellus fulgens and Trichaptum abietinum from natural fruiting bodies and to improve their growth conditions on solid nutrient media. The identification of fungi was performed based on their morphological features and using the Sanger sequencing method. Cultivation was carried out by placing inoculum in the middle of a Petri dish and at the edge, which provided a more comprehensive definition of the characteristics of colonies and fungus hyphae. New strains were registered in Genbank Overview. The optimal cultivation temperature was 27 °C for all studied strains. The highest radial growth was observed on synthetic medium for D. tricolor (5.26 mm/day) and T. abietinum (7.5 mm/day), and on synthetic medium with lignin for P. fulgens (2.98 mm/day). The biomass amount of D. tricolor KS11 was 133.25 mg at 9 days of cultivation, that of P. fulgens KS12 was 86.73 mg at 16 days, and that of T. abietinum KS10 was 227.33 mg at 6 days. New strains of fungi can be used to obtain biologically active substances for the food, pharmaceutical and cosmetic industries.

Source: BioTech

DOI: 10.3390/biotech14020030

Authors: Maria Alexandrovna Sysoeva, Ilyuza Shamilevna Prozorova, Elena Vladislavovna Sysoeva, Tatyana Vladimirovna Grigoryeva, Ruzilya Kamilevna Ismagilova

Institution: Kazan Federal University

Language: English

Topic: Fungal Biology and Applications

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDGs: Zero hunger, Responsible consumption and production

Open Access status: gold

Estimated APC paid: $1,930