Advanced Solid Phase Extraction Tools for Measuring Bempedoic Acid and its Active Metabolite in Human Plasma by Using LC-MS/MS
Year: 2025
Type: article
Abstract: Aim/Background: To simultaneously measure bempedoic acid and its primary metabolite, bempedoic acid metabolite-ESP15228, in human plasma via UPLC-MS/MS, the QuEChERS extraction tactic is not suitable. Instead, employ a tailored bioanalytical approach. Materials and Methods: The QuEChERS technique was employed to extract human plasma, followed by analysis using LC-MS/MS. Separation of compounds was attained using a Zorbax C18 analytical column (50 mm×2.1 mm, 1.7 μm), with retention times of 4.35 and 5.58 min, respectively. The mobile phase consisted of acetonitrile (80%) and 10 mm ammonium acetate (20%), flowing at a rate of 0.80 mL/min. Ionization of the analyte occurred in the TQD
Authors: Mahesh Meghavath, Nayakanti Devanna
Institution: Jawaharlal Nehru Technological University Anantapur
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W4409823309