Duodenal mucosal ablation with irreversible electroporation reduces liver lipids in rats with non-alcoholic fatty liver disease
Year: 2025
Type: article
Abstract: BACKGROUND: Duodenal mucosal ablation (DMA) using irreversible electroporation (IRE) with a glucagon-like peptide-1 receptor agonist has been clinically shown to reduce liver lipid deposition in non-alcoholic fatty liver disease (NAFLD). However, the specific metabolic contributions of DMA using IRE in NAFLD remain unclear. AIM: To assess the feasibility and effectiveness of DMA using IRE in NAFLD rat models. METHODS: Seven-week-old male Sprague-Dawley rats underwent DMA using IRE after 8 weeks on a high-fat diet. Two weeks post-treatment, duodenal and liver tissues and blood samples were collected. We evaluated differences in the duodenal wall structure, liver lipid deposition, enteroendocrine, claudin, and zonula ocludens-1 in the duodenal mucosa. RESULTS: DMA using IRE could be safely performed in rats with NAFLD without duodenal bleeding, perforation, or stenosis. The duodenum healed well 2 weeks after DMA and was characterized by slimmer villi, narrower and shallower crypts, and thicker myenterons compared with the sham-control setting. Liver lipid deposition was reduced and serum lipid index parameters were considerably improved in the DMA setting. However, these improvements were independent of food intake and weight loss. In addition, enteroendocrine parameters, such as claudin, and zonula ocludens-1 levels in the duodenal mucosa, differed between the different settings in the DMA group. CONCLUSION: By altering enteroendocrine and duodenal permeability, simple DMA using IRE ameliorated liver lipid deposition and improved serum lipid parameters in NAFLD rats.
Authors: Jia-Wei Yu, Qi Zhao, Pei-Xi Li, Ya-Xuan Zhang, Bi-Xuan Gao, Lin-Biao Xiang, Xiao-Yu Liu, Lei Wang, Yi-Jie Sun, Ze-Zhou Yang, Yu-Jia Shi, Yun-Fei Chen, Meng-Bo Yu, Hong-Ke Zhang, Lei Zhang, Qin-Hong Xu, Lu Ren, Dan Li, Yi Lyu, Feng-Gang Ren, Qiang Lu
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4409826890