In vitro antimicrobial photodynamic therapy for methicillin-resistant Staphylococcus aureus using flexible organic light-emitting diode
Year: 2025
Type: article
Abstract: • Photodynamic therapy (PDT) against MRSA using high-power OLED and methylene blue (MB). • OLED–PDT effectively inactivated MRSA in liquid, biofilm, and porcine skin. • High-power OLED and MB is a promising method to reduce the risk of bacterial infection. The most critical aspect of managing methicillin-resistant S. aureus (MRSA) infections is preventing their spread by directly controlling MRSA. Studies have revealed that light-emitting diodes (LEDs) or lasers can be used for photodynamic therapy (PDT) to effectively kill MRSA. Organic light-emitting diodes (OLEDs) can also be used as a light source for PDT, but research cases are extremely rare, and most of them have used low-power OLEDs. Therefore, verification studies using high-power OLEDs are required to increase the utilization of OLEDs in PDT applications. In this study, we examined the inactivation effects of PDT on MRSA using a low-voltage/ high-power OLED device. The 632 nm OLED emitted 5–40 mW/cm 2 of light at less than 5 V, and 22 mW/cm 2 of light was selected as the optimal condition for OLED–PDT. The inactivation effects of OLED–PDT with 10 μM methylene blue (MB) against MRSA suspension (39.6 J/cm 2 ), MRSA biofilm (79.2 J/cm 2 ), and MRSA-inoculated porcine skin (39.6 J/cm 2 ) were approximately 86.2%, 73.4%, and 80.2%, respectively. In conclusion, the combination of low-voltage/ high-power OLED and MB could be a promising way to reduce the risk of bacterial infection, contributing to the development of effective portable or wearable medical devices.
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,610
OpenAlex ID: W4409829013