The structure of the microbiota of the colon of young pigs is normal and in the pathology of the gastrointestinal tract
Year: 2025
Type: article
Abstract: Diseases of the digestive organs in animals in the conditions of industrial pork technology are widespread in all technological groups. Diarrhea syndrome is the most frequent in piglets on nursery, especially at the beginning of the period, which is associated with abrupt weaning and diet change. The aim of the work was to carry out a comparative assessment of fecal intestinal microbiota in healthy piglets and saplings with diarrheal syndrome using a molecular genetic method. Biological material was collected at a large pig-breeding complex. The object of the study was healthy and sick young pigs in the technological group of nursery. The real-time polymerase chain reaction (qPCR) method was used as a molecular genetic method. According to the results of laboratory studies, it was found that the development of the disease in animals was accompanied by an increase in the total number of bacteria in the contents of the rectum by 107.32. In the structure of the fecal microbiota, the content of desirable microorganisms in young animals with clinical manifestation of diarrhea was 82.19 %, which is 15.40 % less than in healthy animals. These changes occur due to an increase in the contents of the rectum in sick piglets of representatives of undesirable microorganisms. When diarrhea occurs in piglets, a decrease in the majority of representatives of the normoflora belonging to the genus Lactobacillus spp., Lachnobacterium spp., Clostridium spp., Megasphaera spp., Veillonella spp., Dialister spp. is recorded in the biological material and an increase in the number of opportunistic and pathogenic microorganisms, in particular Peptostreptococcus spp., Mobiluncus spp., Corynebacterium spp., Atopobium spp., Staphylococcus spp., Staphylococcus spp., as well as the Enterobacteriaceae family. Understanding the structural changes of the microbiota in normal and pathological conditions will allow for the correct interpretation of the experiments aimed at correcting the microbiome using probiotic agents aimed at the competitive replacement of opportunistic and pathogenic microflora.
Authors: A. V. Filatov, A. V. Minin, S. V. Nikolaev
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4409831547