Work

Gene expression of transcripts encoding putative secreted proteins from an oil palm fungal pathogen Ganoderma boninense

Year: 2025

Type: article

Abstract: Ganoderma boninense is a white rot fungus which causes basal stem rot (BSR) disease in oil palm and economic loss to the oil palm industry in Southeast Asia. The existing solutions for the disease are ineffective and the genetic determinants responsible for the disease occurrence are understudied. This study reported the characterisation of four transcript sequences encoding metalloproteinase ( U14090 ), aspartic protease ( U42611 ), lipase ( U56931 ), and polysaccharide deacetylase ( U128397 ) from G. boninense . The candidate transcripts were cloned and confirmed by sequencing . The gene expression of U56931 was up-regulated in mycelial cultures at 21- and 28-days after inoculation (dai) whereas the expression of U42611 was down-regulated in mycelial cultures at 28-dai, in comparison to that at 7-dai. Salicylic acid which is involved in plant biotic stress response was shown to be able to down-regulate the gene expression of U56931 . The gene expression of U14090 was up-regulated in G. boninense in contact with oil palm roots for 24- and 48-h post inoculation (hpi) compared to that in the uninoculated control. The findings of this study may facilitate the design of future functional studies and help to prioritise candidate fungal genes for gene editing. In our study, transcripts encoding for putative metalloproteinase ( U14090 ), lipase ( U56931 ), and polysaccharide deacetylase ( U128397 ) were up-regulated in G. boninense in contact with oil palm suggesting their roles in host-pathogen interactions. These candidates may help the pathogen to evade host defence surveillance through the suppression of chitin-induced defence. • Transcripts encoding metalloproteinase, aspartic protease, lipase, and polysaccharide deacetylase were cloned from G. boninense. • All of the transcripts except aspartic protease were up-regulated upon host exposure. • Transcript encoding lipase was enhanced during vegetative growth but suppressed by salicylic acid.

Source: Physiological and Molecular Plant Pathology

DOI: 10.1016/j.pmpp.2025.102715