The Antarctic moss 2-oxoglutarate/Fe(II)-dependent dioxygenases (Pn2-ODD2) enhanced the tolerance to drought and oxidative stress
Year: 2025
Type: article
Abstract: BACKGROUND: Flavonoid biosynthesis pathway is generally thought unique to land plants and has assisted plants to adapt the terrestrial ecosystems. In this pathway, four 2-oxoglutarate/Fe(II)-dependent dioxygenases (2-ODDs), i.e., flavone synthase I (FNSI), flavanone-3-hydroxylase (F3H), flavonol synthase (FLS) and anthocyanin synthase/leucoanthocyanidin dioxygenase (ANS/LDOX), catalyze the hydroxylation and desaturation reactions. In bryophytes, the earliest land plant group, little is known about the biological functions of these enzymes. RESULTS: Here, we cloned a Pn2-ODD2 gene of flavonoid biosynthesis pathway from Antarctic moss Pohlia nutans, which was induced by exogenous NaCl, PEG and abscisic acid (ABA) treatment. Overexpression of Pn2-ODD2 increased the drought resistance in Physcomitrium patens and Arabidopsis thaliana during gametophyte growth and seed germination, respectively. Overexpressed-Pn2-ODD2 Arabidopsis also exhibited the enhanced tolerance to oxidative stress, with the downregulation of ROS generation gene and increased the total flavonoid content. Also, overexpression of Pn2-ODD2 decreased the ABA sensitivity in transgenic P. patens and Arabidopsis. Meanwhile, overexpression of Pn2-ODD2 resulted in an increase in both anthocyanins and flavonols in Arabidopsis, which was correlated with the up-regulated anthocyanin biosynthesis gene. CONCLUSIONS: Taken together, Pn2-ODD2 conferred the resistance to drought and oxidative stress by regulating antioxidant defense system in plants.
Source: BMC Plant Biology
Authors: Huijuan Wang, Han Li, Chaochao Li, Shenghao Liu, Pengying Zhang
Institutions: Shandong University, First Institute of Oceanography
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,090
OpenAlex ID: W4409864175