Work

Suppressiveness of spent mushroom substrate amendment against eggplant Verticillium wilt

Year: 2025

Type: article

Abstract: BACKGROUND: Verticillium wilt, caused by Verticillium dahliae, is a devastating soil-borne disease. Spent mushroom substrate (SMS) has shown potential as a soil amendment for controlling soil-borne diseases. However, the mechanisms underlying its disease-suppressive effects remain poorly understood. Here, the efficacy of SMS in suppressing eggplant Verticillium wilt and mechanisms related to rhizosphere microbiome regulation were investigated. RESULTS: We tested different SMS sources (Pleurotus ostreatus, Hypsizygus marmoreus, Lentinus edodes), particle sizes (45, 75, 150, 300 μm), and addition ratios (0.5-8%, w/w). The fungus control efficacy ranged from 26 to 66%, with best results from 2% SMS of L. edodes at 150 μm. This treatment resulted in 5.7-fold reduction in the Verticillium dahliae population in eggplant rhizosphere. Eggplant fresh and dry weights of shoots and roots, and plant height, significantly increased with 2% SMS amendment. 16S rDNA sequencing revealed alterations in rhizosphere bacterial communities, with an increase in indigenous beneficial bacteria, particularly Bacillus spp., following SMS amendment. Spent mushroom substrate co-inoculated with exogenous biocontrol strain Bacillus subtilis NCD-2 achieved a synergistic effect against Verticillium wilt than both SMS or NCD-2 alone. CONCLUSIONS: Results revealed that SMS protects eggplants against Verticillium wilt, largely by recruiting Bacillus spp. to the rhizosphere. The enrichment effect of indigenous Bacillus spp. in the rhizosphere mediated by SMS similarly applies to Bacillus inoculum, enhancing its efficacy in controlling eggplant Verticillium wilt. These findings enhance our understanding of the protective effects of SMS and its role in the biocontrol of Verticillium wilt. © 2025 Society of Chemical Industry.

Source: Pest Management Science

DOI: 10.1002/ps.8799

Authors: Yuanhang Qu, Xiaomeng Liu, Zhenhe Su, Qinggang Guo, Ping Ma

Institutions: Hebei Agricultural University, Ministry of Agriculture and Rural Affairs, Hebei Academy of Agriculture and Forestry Sciences

Language: English

Topic: Plant Disease Management Techniques

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDGs: Zero hunger, Life on land

Open Access status: bronze

Funder: National Natural Science Foundation of China