Immunohistochemical evidence for hyaline-like cartilage formation after autologous matrix-induced chondrogenesis for osteochondral lesions of the talus: Case reports of 2 patients.
Year: 2025
Type: article
Study design: Case Report
Abstract: OBJECTIVES: ) combines bone marrow stimulation, filling of subchondral defects, and application of a collagen bilayer matrix. The purpose of the study was to analyze the cartilage at the treated defect site in two patients. METHODS: procedure) for failure due to ligamentous instability. During revision, the repair cartilage was evaluated regarding its integration, presence of fissures, thinning, or firmness. Samples for histologic evaluation were taken from the centre of the treated site. The samples were examined using standard histological and immunohistochemistry techniques. RESULTS: During the revision arthroscopy, the regenerated cartilage was the same color but softer than the surrounding cartilage, superficially frayed, without fissures and even with the level of the original cartilage, but not completely stable on the subchondral bone. Histology revealed the presence of Safranin-O-positive fibrocartilage-like tissue. Additionally, cartilaginous-like tissue was found in the 36-month biopsy. IHC revealed a fraction of collagen type II positive cells in the fibrocartilage-like tissue as well as a collagen type II positive extracellular matrix. The cartilaginous tissue of the 36-month biopsy revealed a homogeneous collagen type II stain. CONCLUSIONS: .
Source: PubMed
DOI: 10.48620/87830
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4409905505