Work

Novel PEI-aldehyde conjugates for gene delivery: Promoting chondrogenic differentiation in human mesenchymal stem cells

Year: 2025

Type: article

Abstract: polyplexes successfully promoted the chondrogenic differentiation of hMSCs, as evidenced by the increased expression of chondrogenic markers (SOX9, type-II collagen [COLII], and aggrecan [ACAN]) and proteoglycan deposition in aggregate cultures, while mitigating the low cell viability found with unmodified PEI. These findings suggest that PEIT2T3A is a promising non-viral vector for targeted gene delivery and hMSC-based regenerative medicine applications.

Source: Molecular Therapy — Nucleic Acids

DOI: 10.1016/j.omtn.2025.102551

Authors: Diego Miranda-Balbuena, Alba Ramil-Bouzas, Naiara Doldán-Mata, Junquera López-Seijas, Juan Fafián-Labora, Ibán Lamas-Criado, Jose-Ramón Caeiro-Rey, Paco Fernández-Trillo, Ana Rey-Rico

Institutions: Universidade da Coruña, Complexo Hospitalario Universitario A Coruña, Servicio Gallego de Salud, Instituto de Investigación Biomédica de A Coruña, Universidade de Santiago de Compostela, Complejo Hospitalario Universitario de Santiago

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,900

Funders: Ministerio de Educación, Cultura y Deporte, Ministerio de Ciencia e Innovación, Xunta de Galicia, Agencia Estatal de Investigación, Servicio Gallego de Salud, Ministerio de Universidades

Awards: G1541474743, G1688594452, G2197331969, G2793831607, G5260651846, G6904152975, G7236289310

OpenAlex ID: W4409912170

FWCI: 1.534

Cites: 55

Cited by: 6

Related to: 10