Work

Biophysical and computational insights into lactoferrin-noscapine interaction: Implications for neurodegenerative diseases

Year: 2025

Type: article

Abstract: . Circular dichroism (CD) spectroscopy revealed subtle changes in LTF's secondary structure and a strong binding affinity between LTF and Noscapine, indicative of specific molecular interactions and conformational adjustments. Additionally, in silico studies were performed to complement the experimental findings. Molecular docking studies identified potential binding sites and highlighted key interactions stabilizing the LTF-Noscapine complex. Molecular dynamic (MD) simulation studies demonstrated that structural compactness is well preserved with only minimal structural alterations observed in the protein upon binding of Noscapine. Together, these results provide comprehensive insights into the molecular basis of LTF-Noscapine interaction, with potential implications for therapeutic applications in NDs. This study lays the groundwork for further exploration of Noscapine's potential in combating NDs and other biomedical challenges.

Source: Biomedicine & Pharmacotherapy

DOI: 10.1016/j.biopha.2025.118101

Authors: Anas Shamsi, Moyad Shahwan, Azna Zuberi, Bishwajit Kundu, Mohd Shahnawaz Khan

Institutions: Ajman University, Northwestern University, Indian Institute of Technology Delhi, King Saud University

Language: English

Topic: Protein Interaction Studies and Fluorescence Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,610