Year: 2025
Type: article
Abstract: Cardiac fibroblasts (CF) are key players after myocardial infarction (MI), but their signaling is only incompletely understood. Here we report a first secretome atlas of CF in control (cCF) and post-MI mouse hearts (miCF), combining a rapid cell isolation technique with SILAC and click chemistry. In CF, numerous paracrine factors involved in immune homeostasis are identified. Comparing secretome, transcriptome (SLAMseq), and cellular proteome disclose protein turnover. In miCF at day 5 post-MI, significantly upregulated proteins include SLIT2, FN1, and CRLF1 in mouse and human samples. Comparing the miCF secretome at days 3 and 5 post-MI reveals the dynamic nature of protein secretion. Specific in-vivo labeling of miCF proteins via biotin ligase TurboID using the POSTN promotor mirrors the in-vitro data. In summary, we identify numerous paracrine factors specifically secreted from CF in mice and humans. This secretome atlas may lead to new biomarkers and/or therapeutic targets for the activated CF.
Source: Communications Biology
Authors: Jasmin Bahr, Gereon Poschmann, Andreas Jungmann, Martin Busch, Zhaoping Ding, Jens Vogt, Ria Zalfen, Julia Steinhausen, Arlen Aurora Euan Martínez, Thorsten Wachtmeister, Daniel Rickert, Tobias Lautwein, Christina Alter, Junedh M. Amrute, Kory J. Lavine, Karl Köhrer, Bodo Levkau, Patrick Most, Kai Stühler, Julia Hesse, Jürgen Schrader
Language: English
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $4,190
Awards: Rolle kardialer stromaler Zellen in Regeneration und Heilung nach Myokardinfarkt., West German Genome Center, Zentrale Koordinationseinheit des Next Generation Sequencing Kompetenznetzwerks, Specification and Function of Tissue Resident and Recruited Macrophages in Cardiac Remodeling and Heart Failure
OpenAlex ID: W4409926657