Mechanism of total saponins of Ranunculus ternatus Thunb. in treatment of breast cancer based on liquid chromatography–mass spectrometry and network analysis
Year: 2025
Type: article
Abstract: Objective To explore the mechanism of total saponins of Ranunculus ternatus Thunb. (RT) in the treatment of breast cancer (BC) using liquid chromatography–mass spectrometry (LC-MS) technology and network analysis. Methods The metabolites of RT were detected using LC-MS. Metabolites and targets of RT and BC were identified in different databases, and potential targets and pathways were predicted using protein–protein interaction network and pathway enrichment analyses. A mouse model of BC created by cellular injection and MCF-7 cells were used as research objects for in vivo and in vitro validation experiments to study the anti-BC mechanism of RT. Results A Kyoto Encyclopedia of Genes and Genomes analysis showed that the Janus kinase/signal transducer and activator of transcription signaling pathway might be associated with the anti-BC effects of RT. The in vivo and in vitro experiments showed that Total saponins from RT had a good anti-BC effect that can inhibit the expression of JAK2 and STAT3-related proteins and mRNA, affect the expression levels of serum inflammatory factors tumor necrosis factor-α, interleukin-6, and interleukin-10, inhibit tumor growth, proliferation, and migration, and promote tumor cell apoptosis. Conclusion Total saponins from RT may play a role in BC treatment by regulating the JAK2/STAT3 signaling pathway.
Source: Frontiers in Pharmacology
Authors: Yuanxin Zhang, Shuo Nan, Wei Zhao, Haisheng Xie, Jinxin Miao, Mingsan Miao
Institution: Henan University of Traditional Chinese Medicine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4409942751