Work

Generation of transgene-free genome-edited carrot plants using CRISPR/Cas9-RNP complexes

Year: 2025

Type: article

Abstract: Key MessageWe report a process by which transgene-free, gene-edited carrot plants can be efficiently produced by introducing Cas9 ribonucleoprotein complexes into protoplasts and regenerating carrot plants from those protoplasts.

Source: Plant Cell Reports

DOI: 10.1007/s00299-025-03499-6

Authors: Rajesh Yarra, Patrick J. Krysan

Institution: University of Wisconsin–Madison

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $4,590

Funders: U.S. Department of Agriculture, National Institute of Food and Agriculture

Award: G3586957773

OpenAlex ID: W4409959916

FWCI: 1.828

Cites: 10

Cited by: 7

Related to: 10