Work

Dietary magnesium hydride supplementation positively influences growth performance, intestinal histology, intestinal microbiota, and metabolites in juvenile largemouth bass (Micropterus salmoides)

Year: 2025

Type: article

Abstract: In present study, the survival, growth performance, intestinal histology, intestinal microbiota, and metabolites of largemouth bass ( Micropterus salmoides ) fed with diets supplemented with different concentrations of magnesium hydride (MgH 2 ), containing 0 mg/kg (H0), 10 mg/kg (H10), 20 mg/kg (H20), and 30 mg/kg (H30) hydrogen molecules. Four hundred and eighty juvenile largemouth bass (38.24 ± 2.37) g were cultured for 8 weeks, and the results showed that dietary supplementation with MgH 2 improved fish survival rate (SR), weight gain rate (WGR), and length, while relative fatness was reduced. The best growth effect was observed in the H20 group. The MgH 2 supplementation group also presented significantly increased villus length, basal layer thickness, and goblet cell number. The intestinal microbiota of the MgH 2 supplementation group was significantly different compared to the control group. The relative abundance of Fusobacteriota and Proteobacteria significantly increased in MgH 2 supplementation group, while the abundance of Firmicutes was decreased. Among them, higher relative abundance of anaerobic bacteria such as Romboutsia , Clostridium_Sensu_Stricto_1 , norank_f_ Barnesiellaceae , and Unclassified_o_ Bacteroidales were observed in the MgH 2 supplementation group. In addition, significant changes in the levels of several intestinal metabolites including N(6)-Methyllysine, irinotecan, capsidiol, taurochenodeoxycholate-7-sulfate, prostaglandin F1a, and caffeic acid 3-glucoside was observed in MgH 2 supplementation group. And changes in intestinal microbiota were significantly correlated with changes in metabolite levels. Overall, dietary supplementation with MgH 2 (20 mg/kg hydrogen molecules) exhibits positive effects on M. salmoides farming based on growth performance, intestinal histology, intestinal microbiota, and metabolites explored in the present study. • Dietary MgH 2 supplementation improve survival and growth of Fish. • Dietary MgH 2 supplementation enhanced intestinal health. • Dietary MgH 2 supplementation modulate intestinal microbiota composition. • Dietary MgH 2 supplementation change intestinal metabolite levels. • Correlation between intestinal microbiota and metabolites.

Source: Aquaculture Reports

DOI: 10.1016/j.aqrep.2025.102819