Work

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

Year: 2025

Type: article

Abstract: selective indicator staining was used to label insulin-producing cells through the zinc association in insulin granules, revealing substantial β-cell loss following treatment of islets with pro-inflammatory cytokines for 24 h. These multi-staining protocols effectively capture and quantify the extent of cytokine-induced damage in islets and can be used to evaluate therapeutics designed to prevent β-cell apoptosis in early type 1 diabetes.

Source: Journal of Visualized Experiments

DOI: 10.3791/67996

Authors: Jubril Akolade, Isaac Crossley, Nikki L. Farnsworth

Institutions: Colorado School of Mines, University of Colorado Anschutz

Language: English

Topic: Pancreatic function and diabetes

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Diabetes and Digestive and Kidney Diseases