Work

Detection module to increase the spatial bandwidth product of a microscope

Year: 2025

Type: article

Abstract: Capturing biological specimens at large scales with sub-micron resolution is crucial for biomedical research, but conventional cameras often cannot handle the pixel requirements. While most microscopes use motorized stages to move samples and capture images tile by tile, we propose a method that eliminates the need for sample movement. Our approach integrates a scanning mechanism within the microscope's detection unit, enabling sequential capture of sub-areas of the field of view (FOV) without physically moving the sample. This "remote scanning" method works with all camera-based microscopes, and we demonstrate its effectiveness in both bright-field and epifluorescence microscopy. This technique is ideal for imaging motion-sensitive samples and large biological specimens, as evidenced in this study with millimeter-scale engineered tissues.

Source: Optics Letters

DOI: 10.1364/ol.563495

Authors: Charlie Kersuzan, Aymerick Bazin, Amaury Badon

Institutions: Centre National de la Recherche Scientifique, Institut d’Optique Graduate School, Laboratoire Photonique, Numérique et Nanosciences

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Agence Nationale de la Recherche, Université de Bordeaux

Awards: TECHNOLOGIES FOR MICROENVIRONMENTS ENGINEERING AND DEEP 3D LIVE IMAGING FOR ORGAN ON CHIP, LIght fIeld Matrix Microscopy for long-term volumetric imaging of Organoids