Year: 2025
Type: article
Abstract: Minimal photon fluxes (MINFLUX) nanoscopy has emerged as a transformative advancement in superresolution imaging, enabling unprecedented nanoscale observations across diverse biological scenarios. In this work, we propose, for the first time, that employing high-order vortex beams can significantly enhance the performance of MINFLUX, surpassing the limitations of the conventional MINFLUX using the first-order vortex beam. Our theoretical analysis indicates that, for standard MINFLUX, high-order vortex beams can improve the maximum localization precision by a factor corresponding to their order, which can approach a sub-nanometer scale under optimal conditions, and for raster scan MINFLUX, high-order vortex beams allow for a wider field of view while maintaining enhanced precision. These findings underscore the potential of high-order vortex beams to elevate the performance of MINFLUX, paving the way towards ultra-high resolution imaging for a broad range of applications.
Source: Light Science & Applications
Authors: Xiao-Jie Tan, Zhiwei Huang
Institutions: National University of Singapore, Suzhou Research Institute
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,190
OpenAlex ID: W4410082484