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Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia

Year: 2025
Type: article
Abstract: Here, we present a protocol that models peripheral artery disease using the surgical induction of hindlimb ischemia (HLI) in mice. We list the steps to surgically excise the femoral artery and assess blood flow recovery through laser Doppler imaging. We then detail procedures for visualizing immune cell recruitment and blood vessel growth to explore the role of inflammatory macrophages in angio/arteriogenesis. For complete details on the use and execution of this protocol, please refer to Mantsounga et al. 1
DOI: 10.1016/j.xpro.2025.103783
Authors: Sheila Sharma, Julia Pierce, Celia A. Butler, Olivya Caballero, Chris Mantsounga, Alan R. Morrison
Language: English
Open Access status: gold
Estimated APC paid: $1,550
Funders: U.S. Department of Veterans Affairs, American Heart Association, Biomedical Laboratory Research and Development, VA Office of Research and Development, Rhode Island Foundation, National Institutes of Health, Clinical Science Research and Development, National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences
Awards: Center for Cancer Signaling Networks, G1520763180, G1834523820, CardioPulmonary Vascular Biology COBRE, G3053335973, G3138906405, G6234278068, G792016239, G8182537113, G9000180973
OpenAlex ID: W4410084282
FWCI: 0.254
Cites: 1
Cited by: 1
Related to: 10