Work

Longitudinal calcium imaging of BNST neurons in mice during optogenetic manipulation of paraventricular thalamus axon terminals

Year: 2025

Type: article

Abstract: Here, we present a protocol to study neural circuits between the paraventricular thalamus (PVA) and the bed nucleus of the stria terminalis (BNST) in mice by combining calcium imaging with optogenetic stimulation of axon terminals. We describe steps for delivering GCaMP6f and ChrimsonR viruses and implanting the gradient-index (GRIN) lens for use with an Inscopix microscope. We then detail procedures for single-neuron tracking over an extended period through longitudinal recording and data smoothing and processing to enhance analysis.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103800

Authors: Selomon Assefa Mindaye, Wei-Hsin Chen, Shih-Che Lin, Chien-Chang Chen

Institutions: Institute of Biomedical Sciences, Academia Sinica, Academia Sinica, National Taiwan University

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550