Work

Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli

Year: 2025

Type: article

Abstract: OBJECTIVES: This study aims to assess whether a fed-batch-based auto-induction method can enhance active hydrogenase production, encompassing cofactor formation and space-time yield. RESULTS: The recombinant Escherichia coli strain BQF8RH8, possessing plasmids for assembly and proper maturation of Cupriavidus necator regulatory hydrogenase (RH), was cultivated in the fed-batch like EnPresso B medium with an autoinduction lactose-based system. In contrast to classical IPTG induction previously performed, we obtained active RH demonstrating the feasibility of the process for active hydrogenase production. CONCLUSION: Our results affirm the viability of the previously developed auto-induction strategy also for a functional hydrogenase with the complex maturation process. This significantly accelerates the process and enhances hydrogenase productivity.

Source: Biotechnology Letters

DOI: 10.1007/s10529-025-03594-4

Authors: Francisco de la Fuente-Kratzborn, Qin Fan, Peter Neubauer, Matthias Gimpel

Institution: Technische Universität Berlin

Language: English

Topic: Metalloenzymes and iron-sulfur proteins

Subfield: Renewable Energy, Sustainability and the Environment

Field: Energy

Domain: Physical Sciences

SDGs: Affordable and clean energy, Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,890