Work

Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique

Year: 2025

Type: article

Abstract: Dermal fibroblasts play a central role in the production and remodeling of dermal extracellular matrix. Their spatially distinct subpopulations exhibit different functions during skin homeostasis, wound healing, and other diseases such as fibrosis and cancer. Here, we present a protocol for detecting 3D dermal fibroblast cellular dynamics in mice without fixing the tissue using an intravital imaging technique. We describe steps for preparing, labeling, and mounting mice. We then describe detailed procedures for intravital imaging, recovery, and quantification. For complete details on the use and execution of this protocol, please refer to Rognoni et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103801

Authors: Toru Hiratsuka, Emanuel Rognoni

Institutions: Osaka International Cancer Institute, The University of Osaka, Queen Mary University of London, Blizard Institute

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550