Sustainable validated methodology for enzymeless determination of Tyrosine using 3D-printed PLA/CB electrodes in synthetic urine
Year: 2025
Type: article
Abstract: Tyrosine (TYR), an amino acid, is a biomarker for several diseases, making its quantification in biological samples clinically relevant. However, conventional gold-standard methods typically require expensive reagents, are time-consuming, and are often not portable. In addition, in most cases, TYR assays are not validated. To circumvent these inconveniences, we present a validated and sustainable methodology for quantifying TYR in synthetic urine (proof-of-concept) on a portable 3D-printed platform. This work evaluated and achieved promising results in the use of 3D printing in the development of a miniaturized and portable analytical method, which explored aspects of analytical validation. The proposed method was optimized for square wave voltammetry parameters and better background electrolytes, resulting in the following optimized conditions: 8 Hz frequency, 50 mV modulation amplitude, 6 mV pulse increment, and sodium acetate buffer (pH 4.5). Validation, performed according to EURACHEM, IUPAC and ICH guidelines, evaluated key performance indicators such as selectivity (via matrix effects and Youden's calibration study), linearity, limits of detection and quantification, precision, and trueness. The method demonstrated adequate selectivity and linearity over the working range of 2 to 10 µmol/L ( p > 0.05). At 6 µmol L −¹ , it showed good repeatability ( n = 6) and intermediate precision ( n = 12), with an RSD (%) of less than 16%. Recovery tests evaluated accuracy using spiked samples at 2, 6, and 10 µmol L − ¹ ( n = 3), with recoveries below 106%. In addition, the proposed method achieved a high greenness score of 0.79, calculated using the AGREE software, where higher scores indicate more environmentally friendly methods. Overall, the proposed method is a low-cost, sustainable, environmentally friendly, portable, and accessible approach for TYR analysis in biological samples.
Source: Talanta Open
Authors: Amanda Morais, Diego Pessoa Rocha, Maria Lurdes Felsner, Andressa Galli
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,190
OpenAlex ID: W4410109370