Work

Assessing Microprocessor complex mutations with a Microsensor system

Year: 2025

Type: article

Abstract: The Microprocessor complex, consisting of DROSHA and DGCR8, is essential for miRNA maturation and gene regulation. Mutations in these proteins are associated with Wilms tumor (WiT), a common pediatric kidney cancer. To explore the impact of these mutations on WiT pathogenesis, we developed the Microsensor system, a novel tool for dynamically monitoring Microprocessor activity in human cells. Using this system, we engineered HEK293T cells to express the DGCR8-E518K mutation, which was previously identified in WiT patients. Our results show that this mutation significantly impairs the Microprocessor's ability to process specific pri-miRNAs in vitro and alters the miRNA expression profiles. This study demonstrates the utility of the Microsensor system in investigating the molecular mechanisms underlying mutations related to the Microprocessor complex.

Source: RNA

DOI: 10.1261/rna.080338.124

Authors: Sheng Bao, Thi Nhu-Y Le, Cong Truc Le, Le Nguyen Bao Tran, Tuan Anh Nguyen

Institutions: Hong Kong University of Science and Technology, HKUST Shenzhen Research Institute

Language: English

Topic: MicroRNA in disease regulation

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W4410119827

FWCI:

Cites: 54

Cited by:

Related to: 10