Temporal dynamics of gene expression during the development of Campylobacter jejuni biofilms
Year: 2025
Type: article
Abstract: Campylobacter jejuni, an important pathogen of bacterial gastrointestinal infections, forms biofilms that enable its survival in different environments. C. jejuni biofilm development is still poorly understood, and thus, in this study, we characterized gene expression changes at different biofilm stages using RNA sequencing. Early biofilms (after 16 and 24 h) showed increased expressions of genes involved in cysteine and methionine metabolism, whereas mature biofilms (after 48 and 72 h) showed decreased expression of genes encoding capsular polysaccharides and lipooligosaccharides. Both early and mature biofilms showed increased expressions of genes involved in flagella formation, leucine metabolism and the oxidative stress response and decreased expressions of genes involved in energy metabolism, iron acquisition and transmembrane transport. This study provides insights into the molecular mechanisms underlying C. jejuni biofilm maturation, environmental resistance and the dynamic nature of gene expression during biofilm development.
Source: Microbial Genomics
Authors: Manca Volk, Ozan Gundogdu, Anja Klančnik
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,839
Awards: G1297351286, G3174946678, Microplastics as a vector of pathogens contamination, infection and resistance: a case of Campylobacter in poultry production and processing, Microbiology and biotechnology of food and environment, Intra-biofilm dynamics of Campylobacter with other bacteria: effects on biofilm formation and composition with a view to the design of innovative control strategies, Understanding the role of the Type II MLA system in OMV biogenesis: towards developing an effective vaccine against Campylobacter jejuni in poultry
OpenAlex ID: W4410120609