Work

Critical size defect in a rat calvaria model using trephination: An animal model for investigating potential bone regenerative scaffolds

Year: 2025

Type: article

Abstract: investigation in translating bioregenerative material into human clinical trials. The critical size defect (CSD) in a rat's calvaria is a popular methodology for demonstrating and investigating the regenerative properties of a biomaterial. Various surgical approaches have been discussed in the literature on creating CSD and can vary based on species, age and anatomical location. This paper aims to provide a reliable and reproducible step-by-step guide for creating a critical-size defect on the calvaria of a laboratory rat using trephination. This paper will:•Cover the surgical approaches and management in the pre-, intra- and post-operative phases.•Provide figures to help visually guide readers in carrying out the operation.•Cover basic general anaesthetic issues and resolutions.

Source: MethodsX

DOI: 10.1016/j.mex.2025.103355

Authors: Jeffrey Huang, Jithendra Ratnayake, George J. Dias

Institution: University of Otago

Language: English

Topic: Bone Tissue Engineering Materials

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,260