Work

Hydrolysis of flavanones from orange peel and evaluation of anticancer potential using naringinase immobilized on corn cob powder

Year: 2025

Type: article

Abstract: The management of waste from global orange production represents an environmental and economic challenge. In this study, the extraction of flavanones from orange peel albedo was optimized as a sustainable and economical alternative to purified substrates. The extracts obtained, rich in naringin , narirutin , hesperidin , neohesperidin and rutin , were enzymatically hydrolyzed by the naringinase complex, whose α-L -rhamnosidase and β-D -glucosidase activities transformed these compounds into prunin and naringenin . Their effect was evaluated in cell viability assays in the SW480 line, identifying compounds with potential biological activity in colon cancer prevention. Purification of naringinase increased its affinity for α-L -rhamnosidase activity, while its immobilization in corn cob powder allowed improving its reusability and stability, retaining up to 75 % of its initial activity after 10 consecutive cycles. By using whole extracts without purification, this study proposes a more viable and scalable approach for the valorization of citrus waste for biological purposes.

Source: Food Bioscience

DOI: 10.1016/j.fbio.2025.106752