Work

lncRNA BBOX1-AS1 regulates the miR-382-5p/CBX3 Signalling pathway to affect the proliferation and apoptosis of glioblastoma cells

Year: 2025

Type: article

Abstract: OBJECTIVE: This study aims to investigate the role of long noncoding RNA (lncRNA) BBOX1-AS1 in regulating the miR-382-5p/CBX3 axis and its impact on glioblastoma cell proliferation and apoptosis. METHODS: U-87 MG glioblastoma cells were divided into the following groups: control, si-NC, si-BBOX1-AS1, si-BBOX1-AS1 + inhibitor NC, si-BBOX1-AS1 + miR-382-5p inhibitor, miR-NC, miR-382-5p mimic, miR-382-5p mimic+pc-NC, and miR-382-5p mimic+pc-CBX3. The expression levels of lncRNAs BBOX1-AS1, miR-382-5p, and CBX3 were detected via qRT-PCR. Cell proliferation was assessed via MTT assays. Apoptosis rates and caspase 3 activity were measured via flow cytometry and caspase 3 activity assay kits. Western blotting was used to evaluate CBX3 protein expression. Dual-luciferase reporter assays were conducted to verify the relationship between the lncRNA BBOX1-AS1 and miR-382-5p. A similar assay was performed for miR-382-5p and CBX3. RESULTS: Compared to normal human astrocytes (HA1800), glioblastoma cells (T98G, HS 683, U251, and U87 MG) exhibited higher levels of lncRNA BBOX1-AS1 and CBX3, along with lower levels of miR-382-5p (P < 0.05). Compared with the si-NC cells, the U-87 MG cells in the si-BBOX1-AS1 group presented reduced lncRNA BBOX1-AS1 expression, proliferation ability, and CBX3 protein expression but increased miR-382-5p levels, apoptosis rates, and Caspase 3 activity (P < 0.05). Compared with the si-BBOX1-AS1 + inhibitor NC group, the si-BBOX1-AS1 + miR-382-5p inhibitor group presented decreased miR-382-5p levels, apoptosis rates, and Caspase 3 activity but increased CBX3 protein expression and proliferation ability (P < 0.05). Compared with the control, the miR-382-5p mimic reduced CBX3 protein expression and proliferation ability while increasing the apoptosis rate and caspase 3 activity (P < 0.05). However, overexpression of CBX3 (miR-382-5p mimic+pc-CBX3) reversed the effects of the miR-382-5p mimic (P < 0.05). Dual-luciferase assays confirmed the relationships between lncRNA BBOX1-AS1 and miR-382-5p and between miR-382-5p and CBX3 (P < 0.05). CONCLUSION: Knockdown of lncRNA BBOX1-AS1 inhibits U-87 MG cell proliferation and promotes apoptosis by upregulating miR-382-5p and downregulating CBX3 expression.

Source: International Immunopharmacology

DOI: 10.1016/j.intimp.2025.114790

Authors: Bo Tan, Tao Chen, Peng Song, Feng Lin, Shuangyin He, Xiaohong Yin

Language: English

Topic: Cancer-related molecular mechanisms research

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,220

Funder: Wu Jieping Medical Foundation