Heat shock protein mediates the fumigation activity of terpinen‐4‐ol and limonene against Tribolium confusum
Year: 2025
Type: article
Abstract: BACKGROUND: Terpinen-4-ol and limonene are important active components used to control storage pests, comprising an effective strategy to reduce the utilization of chemical pesticides and postpone the development of resistance. RESULTS: Terpinen-4-ol and limonene had significant fumigation activity against Tribolium confusum, which showed positive association with temperature. Two heat shock protein genes (HSPs) (TcfHSP16.8 and TcfHSP19.0) were selected as candidate targets according to RNA-sequencing data. Following RNA interference-mediated silencing of these two genes, T. confusum showed no notable disparity in eclosion rates between temperatures of 28 °C and 40 °C. Subsequent sensitivity assessments using 30% lethal concentrations demonstrated mortality rates of 58.41% (TcfHSP16.8) and 57.10% (TcfHSP19.0) with terpinen-4-ol, and 58.62% (TcfHSP16.8) and 55.95% (TcfHSP19.0) with limonene at 40 °C, respectively. Thus, silencing TcfHSP16.8 and TcfHSP19.0 increased the susceptibility of T. confusum to both compounds at 40 °C, with limonene showing a more pronounced effect. CONCLUSION: These results suggested that HSPs mediate and enhance the sensitivity of T. confusum to terpinen-4-ol and limonene, which is important to improve the control to storage pests and reduce the utilization of chemical pesticides. © 2025 Society of Chemical Industry.
Source: Pest Management Science
DOI: 10.1002/ps.8879
Authors: Su Shi, Yuzhi Zhang, Rui Xing, Min Liao, Hailong Wu, Yong Huang, Quan Gao, Haiqun Cao
Institution: Anhui Agricultural University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W4410138737