Work

Array Assembler Provides Greatly Simplified crRNA Array Design for CRISPR Cas12 and Cas13 Variants

Year: 2025

Type: article

Abstract: As newer CRISPR variants have emerged and corresponding toolkits have been developed, researchers can now readily target multiple genes simultaneously for knockout, activation, or repression alongside being able to bind or cleave mRNA. However, as larger multitargeting crRNA arrays are required for these experiments, the design process becomes more complicated, taking more time and increasing risks of errors being introduced. The Array Assembler seeks to address the critical bottleneck that emerges during longer crRNA array design by providing a highly user-friendly tool to process input crRNA spacer sequences into the oligos required for efficient assembly of the corresponding crRNA array. By enabling rapid and reliable design of oligos for efficient assembly of crRNA arrays from a user-defined list of crRNA spacer sequences this tool should prove useful for a wide range of laboratories employing genomic perturbations.

Source: ACS Synthetic Biology

DOI: 10.1021/acssynbio.5c00100

Author: James W. Bryson

Institution: University of Copenhagen

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,500

Funders: Lundbeckfonden, H. Lundbeck A/S