Year: 2025
Type: article
Abstract: Lanthanide binding tags (LBTs) stand out as a prominent group of fluorescent probes that are extensively utilized in biological detection. However, research on LBTs has predominantly emphasized their fluorescence properties, which frequently compromised by background fluorescence noise. Investigating magnetic properties could optimize detection methodologies that offer enhanced sensitivity and specificity. This study measures the response of a relaxometer based on ensemble nitrogen‐vacancy (NV) centers in diamond to various amounts of LBTs with gadolinium ions, determining the detection limit of LBTs to be 25 fmol. A detection scheme employing the NV relaxometer to detect specific binding between LBTs and target is then proposed and demonstrated. Specifically, the study assesses the relaxometer's response to various concentrations of the interaction between the modified LBTs and Receptor‐Binding Domain (RBD) of SARS‐COVID‐2 spike protein, with the detection threshold reaching ≈2 pmol. The research provides a potential application platform for biomarker detection under picomole concentration by using NV centers to detect the magnetism of LBTs.
Source: Advanced Optical Materials
Authors: Zibo Gao, Zhengzhi Jiang, Qiyu Liang, Ruihua He, Van Cuong Mai, Yingwei Tang, Qirong Xiong, Wenting Zhao, Hongwei Duan, Hongliang Sun, Mo Li, Yansong Miao, Weibo Gao
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W4410177438