Immunohistochemical Study on Schistosoma Mansoni Induced Liver Fibrosis in Experimentally Infected Mice
Year: 2025
Type: article
Abstract: In chronic schistosomiasis, eggs trapped in the liver triggered granulomatous immunopathological lesions which leading to severe liver fibrosis. The study checked the impact of IL17A on serum IL-13 level, TNF-α and TGF-1β protein expressions on S. mansoni induced liver fibrosis in experimentally infected mice. Forty four male C57BL/6 pathogen-free mice obtained, divided into 4 groups: GI negative control, GII positive control infected with 30±5 cercariae, GIII infected/anti-IL-17 mAb was given, GIV isotype-matched IgG2a mAb was given as a challenge, were sacrificed at 6, &10 weeks postinfection, then underwent liver enzymes and cytokines assessment, histopathological and immunohistochemical studies. The results showed that the profibrogenic activity of IL 17 was blocked by anti/IL-17 monoclonal antibodies with significant decrease in IL 13, liver enzymes, pro-inflammatory cytokines and granuloma size. Also, reduced TGF- β1 expression, and increase TNF-α expression levels in livers of S. mansoniinfected mice in the later stages, inducing its anti-fibrotic effects. So may be targeted in Schistosoma egg induced liver fibrosis as potential immunotherapeutic agent.
Authors: MOHAMMED S. ELFARAMAWY, AHMED A. E. ELBEHERY, HAITHAM KH. AHMAD, MOAMEN A. I. MAZEN, MOHAMED A. AMIN
Institution: Al-Azhar University
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4410180844