Work

Hyaluronic Acid‐Based Bleaching Gels With NF_TiO 2 and Violet LED : Efficacy and Cytotoxicity of Low‐Concentration H 2 O 2 for In‐Office Bleaching

Year: 2025

Type: article

Abstract: ABSTRACT Objective To evaluate the efficacy and cytotoxicity of in‐office bleaching gels with hyaluronic acid (HA) or carbomer 940 (CAR), titanium dioxide nanoparticles co‐doped with nitrogen and fluorine (NF_TiO 2 ), and hydrogen peroxide (HP; H 2 O 2 ) at 1.5% and 6% with violet LED irradiation. Materials and Methods 48 bovine enamel/dentin discs (5 × 3 mm) stained with black tea for 24 h were assigned to six groups ( n = 8): HA‐1.5%HP + LED, HA‐6%HP + LED, CAR‐1.5%HP + LED, CAR‐6%HP + LED, 35%HP‐commercial (control), and a negative control (no treatment). The discs were placed in artificial pulp chambers (APCs) and underwent three 30‐min bleaching sessions with 20 violet LED cycles (1‐min activation, 30‐s pause) at 7‐day intervals. Extracts were applied to MDPC‐23 cells, assessing color change (ΔE 00 ), whiteness index (ΔWI D ), H 2 O 2 diffusion, cell viability (CV), oxidative stress (OxS), and cell morphology (SEM). Data were analyzed by one‐way ANOVA and Tukey post hoc test ( α = 0.05). Results Gels with HA showed no statistical difference in ΔE 00 and ΔWI D compared with 35%HP‐commercial ( p > 0.05). H 2 O 2 diffusion and oxidative stress were lower in 1.5% and 6% HP groups. Cell viability was higher in 1.5% HP groups ( p < 0.05). There were no changes in cell morphology. Conclusion Bleaching gels with HA, NF_TiO 2 NPs, low H 2 O 2 concentrations, and violet LED irradiation reduced cytotoxicity without compromising efficacy. Clinical Relevance Experimental bleaching gels with hyaluronic acid, NF_TiO 2 nanoparticles, low H 2 O 2 concentrations, and combined with violet LED irradiation achieve similar efficacy to high‐H 2 O 2 gels (35%). This approach also promises to reduce cytotoxic damage, providing a safer in‐office bleaching option.

Source: Journal of Esthetic and Restorative Dentistry

DOI: 10.1111/jerd.13484

Authors: Marcos Roberto de Lima de Benati, Priscila Borges Gobbo de Melo, Rafael Antonio de Oliveira Ribeiro, Fernando Luis Esteban Florez, Carlos Alberto de Souza Costa, Vanessa Cavalli

Institutions: Universidade Estadual de Campinas (UNICAMP), Universidade Estadual Paulista (Unesp), University of Oklahoma Health Sciences Center

Language: English

Topic: Dental Erosion and Treatment

Subfield: Orthodontics

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,030