Centrally administered prostaglandin E2 suppresses the micturition reflex in rats
Year: 2025
Type: article
Abstract: Prostaglandin E 2 (PGE 2 ) facilitates the micturition reflex at the lower urinary tract and spinal cord levels. However, the roles of brain PGE 2 in reflex regulation remain unclear. Therefore, we aimed to investigate the effects of intracerebroventricularly administered PGE 2 on the micturition reflex. We further investigated whether the PGE 2 -induced responses were dependent on the sympathetic nervous system (SNS) and identified the brain E-prostanoid receptor subtypes (EP 1 –EP 4 ) involved in PGE 2 -induced effects. Intracerebroventricularly administered PGE 2 (0.1, 0.3, or 1 nmol/rat) dose-dependently increased the intercontraction intervals (ICI) and threshold pressure required to induce micturition (TP) without altering maximal voiding pressure in urethane-anesthetized (0.8 g/kg, ip) male rats. PGE 2 (1 nmol/rat) significantly increased the mean blood pressure; 6-hydroxydopamine-induced chemical sympathectomy ameliorated this increase. In contrast, chemical sympathectomy had no significant effect on the PGE 2 -induced increases in ICI and TP. Intracerebroventricularly pretreated SC51322 (EP 1 receptor antagonist, 100 nmol/rat) and PF04418948 (EP 2 receptor antagonist, 100 nmol/rat), but not L-798106 (EP 3 receptor antagonist, 100 nmol/rat) or L-161982 (EP 4 receptor antagonist, 100 nmol/rat), significantly attenuated the PGE 2 (1 nmol/rat)-induced changes in ICI and TP. These results suggest that centrally administered PGE 2 suppresses the rat micturition reflex through brain EP 1 and EP 2 receptors, independent of SNS activation.
Institutions: Kōchi University, University of Kochi
Language: English
Subfield: Urology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,070
OpenAlex ID: W4410193375