Assessment of genomic instability in operating room staff using cytokinesis block micronucleus assay
Year: 2025
Type: article
Study design: Observational Study
Abstract: Introduction: Anesthetic gases are the main pollutants in operating rooms (OR).Aim: In this study, we explored the genomic instability and nuclear division index (NDI) in OR staff with prolonged exposure to low doses of waste anesthetic gases (WAGs).Material and Methods: Study groups were: total 40 participants, 20 were OR staff (comprising 17 anesthesia technicians and three nurses from OR), while 20 that were not exposed to WAGs were assigned as control.The cytokinesis-blocked micronucleus assay (CBMN) was applied to assess genomic instability.Indicators of genomic instability were micronucleus (MN), nuclear bud (NBUD), and nucleoplasmic bridge (NPB).NDI was used as indicator of cell kinetic.Results: The MN and NBUD average frequencies were significantly elevated in the operating room staff when compared to the respective controls (p<0.001,p<0.005).On the other hand, NPB and NDI showed no significant differences in both groups (p>0.05).Among the OR staff, a positive association in terms of correlation (r=0.57,p<0.01) is reported between age and MN frequency.Similarly, total years of service and MN frequency (r=0.47,p<0.05) were correlated.No differences in gender of operating room employees were found in MN, NPB, and NBUD frequencies (p>0.05).Conclusion: Our results present no significant impact of WAG exposure on cell kinetics but significantly increased the frequency of MN and NBUD.
Authors: Topaloğlu Nur, Hayal Çobanoğlu
Institution: Çanakkale Onsekiz Mart Üniversitesi
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: diamond
Awards: G1002539156, G1321993874, G4903128616
OpenAlex ID: W4410208473