Protocol for in-plate staining of clinical peripheral blood mononuclear cell samples for high-throughput analysis via spectral flow cytometry
Year: 2025
Type: article
Abstract: Clinical trial research relies on robust assays to monitor immune responses in patients. We present a protocol for optimization of in-plate staining for spectral flow cytometry tailored for high-throughput analysis of peripheral blood mononuclear cell (PBMC) samples from clinical research correlative studies. We describe steps for antibody titration, optimal in-plate staining, data acquisition on Sony ID7000 spectral cell analyzer, and validation of a flow cytometry assay.
Source: STAR Protocols
Authors: Isabella Marciello, Sonya Feng, Ilana Gomez Diaz, Julia Huezo, F. Stephen Hodi, Maria Cimpean, Joanna Baginska
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,550
OpenAlex ID: W4410224921