Year: 2025
Type: article
Abstract: A linker unit was designed and synthesized that can serve both as a hairpin turn in a DNA duplex and anchor point for an aromatic helical foldamer mimicking the shape and surface properties of B-DNA. Methods were developed to synthesize natural/non-natural chimeric molecules combining foldamer and DNA segments. The ability of the linker to position the foldamer helix and the duplex DNA so that their rims and grooves are in register, despite their completely different chemical nature, was demonstrated using single crystal X-ray diffraction, circular dichroism and molecular models. Bio-layer interferometry confirmed that artificial hairpin DNA duplexes keep their ability to bind to DNA binding proteins. The chimeric molecules may pave the way to competitive inhibitors of protein-DNA interactions involving sequence-selective DNA-binding proteins.
Institution: Ludwig-Maximilians-Universität München
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,510
Funders: Fonds der Chemischen Industrie, European Commission, Deutsche Forschungsgemeinschaft, Bundesministerium für Bildung und Forschung, HORIZON EUROPE Framework Programme, Deutsches Elektronen-Synchrotron, Horizon 2020, Elitenetzwerk Bayern, H2020 Marie Skłodowska-Curie Actions, HORIZON EUROPE European Research Council
Awards: G1487475300, TRR 237: NukleinsäureImmunität, G3251515213, SFB 1361: Regulation von DNAReparatur und Genomstabilität, G3726397627, G4102717101, G434337789, SFB 1309: Chemische Biologie epigenetischer Modifikationen, Nucleic Acids for Future Gene Editing, Immunotherapy and Epigenetic Sequence Modification, CNATM: Chemisch modifizierte Nukleoside, Nukleotide und Oligonukleotide als Therapeutika und Vakzine (Verbundprojekt 1) - A, G6354276587, G7255431040, G7580957167, Nucleic Acids for Future Gene Editing, Immunotherapy and Epigenetic Sequence Modification, G7886024558, G82931992, G8483229536
OpenAlex ID: W4410240866