1,25-dihydroxyvitamin D in the elderly population: Comparison of liquid chromatography tandem mass spectrometry and CLIA immunoassay (LIAISON®XL) methods
Year: 2025
Type: article
Study design: Observational Study
Abstract: Background 1α,25-dihydroxyvitamin D is the biologically active form of vitamin D 3 (cholecalciferol) and vitamin D 2 (ergocalciferol). The determination of 1,25(OH) 2 D is clinically relevant in the diagnostics of vitamin D metabolism disorders, PTH-independent hypercalceamia and hypophosphatemic syndromes. The quantitative assessment of 1,25(OH) 2 D may be a challenge since it circulates in picomolar concentrations in the blood. Aims of the study Comparison of two methods: chemiluminescent immunoassay (CLIA, LIAISON®XL) and Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) for determinations of 1,25(OH) 2 D concentrations in elderly populations. The secondary aim was to assess correlation between 1,25(OH) 2 D for CLIA LIAISON®XL and LC-MS/MS methods and selected factors (vitamin D metabolites, calcium, albumin, PTH, creatinine concentrations in serum and age). Materials and methods The study was conducted on 54 patients aged from 60 to 96 at the Bielański Hospital in Warsaw, Poland. The determination of 1,25(OH) 2 D using CLIA LIAISON®XL and LC-MS/MS methods was performed. Results Both methods (CLIA LIAISON®XL immunoassay and LC-MS/MS technique) were strongly positively correlated (r=0.86; p<0.001). In the LC-MS/MS technique, concentration of 1,25(OH) 2 D was significantly higher compared to the CLIA LIAISON®XL immunoassay. The regression equation revealed method interchangeability. Concentration of 1,25(OH) 2 D was significantly correlated with various basic biochemical parameters (albumin and calcium levels) for both methods. Conclusions In our study, measurement of 1,25(OH) 2 D using CLIA LIAISON®XL was not inferior to LC-MS/MS measurement. The assessment of 1,25(OH) 2 D using CLIA LIAISON®XL , characterized by short turnaround time, low costs and high accuracy, may be an optimal choice for elderly patients who often require prompt diagnosis and treatment.
Source: Practical Laboratory Medicine
Authors: Dorota Leszczyńska, Alicja Szatko, Magdalena Ostrowska, Magdalena Zgliczyńska, Konrad Kowalski, Wojciech Zgliczyński, Piotr Glinicki
Language: English
Topic: Vitamin D Research Studies
Subfield: Pathology and Forensic Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,140
OpenAlex ID: W4410246068