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Mycobacterium smegmatis Ribosome Purification, Co-sedimentation, and Subunit Association Assay

Year: 2025

Type: article

Abstract: using an optimized sonication cycle. • Detection of ribosome-protein interactions through density gradient ultracentrifugation. • Simple steps to obtain tRNA-free 70S ribosomes that are crucial for several biochemical experiments. • Screening of proteins or ligands for their ribosomal anti-association activity.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5318

Authors: Aneek Banerjee, Sandip Dey, Krishnamoorthi Srinivasan, Ankit Dhur, Priya Baid, Jayati Sengupta

Institutions: Indian Institute of Chemical Biology, Academy of Scientific and Innovative Research

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W4410247583

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