Year: 2025
Type: article
Abstract: -acetylglucosamine (UDP-GlcNAc) on 100 mg scale, with capacity for multiple reuses. The modular continuous flow approach described here represents a significant, up to 11-fold, improvement in space time yield (STY) when compared to batch studies, along with preventing product induced enzyme inhibition, reducing the need for an additional enzyme to break down inorganic pyrophosphate (PPi). The modular nature of the system has also allowed tailored conditions to be applied to each enzyme, overcoming issues relating to thermal stability. This development presents a platform approach towards a more efficient, continuous synthesis of important glycan targets including glycoproteins, specific oligosaccharide sequences and glycosylated drug targets.
Source: Reaction Chemistry & Engineering
DOI: 10.1039/d5re00127g
Institution: Keele University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4410247910