Work

Potential shortening of testing time by combination of loop-mediated isothermal amplification and enrichment culture for detection of Salmonella spp. in cattle farms

Year: 2025

Type: article

Abstract: To prevent the spread of bovine salmonellosis on farms, minimizing the detection time is an effective approach. At present, Salmonella spp. is typically detected via conventional culture methods involving an enrichment culture process taking several days. We attempted rapid detection of individual feces and environmental samples spiked with Salmonella Typhimurium or S. Dublin employing a loop-mediated isothermal amplification kit for detecting Salmonella spp. Notably, in comparison with conventional culture methods, the use of 18-hr enrichment cultures as samples can detect Salmonella spp. in feces and environment at least 24 and 48 hr earlier. Additional validation using clinical samples is necessary.

Source: Journal of Veterinary Medical Science

DOI: 10.1292/jvms.24-0291

Authors: Megumi ITOH, Masato TAKENAKA, W.P.P.S.I. PERERA, Yoshie SAKURAI, Chihiro AIKAWA, Masashi OKAMURA

Institutions: Obihiro University of Agriculture and Veterinary Medicine, University of Peradeniya, Hokkaido Research Organization

Language: English

Topic: Salmonella and Campylobacter epidemiology

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: diamond

OpenAlex ID: W4410336277

FWCI: 0.44

Cites: 22

Cited by: 1

Related to: 10