Antimicrobial Effect of Cinnamaldehyde and α ‐Terpineol on Endodontic Biofilms of Candida albicans and Enterococcus faecalis
Year: 2025
Type: article
Abstract: Objectives: The objective was to assess the antimicrobial effect of cinnamaldehyde and α ‐terpineol on mono‐species and dual‐species biofilms involved in endodontic infection. Materials and Methods: The phytoconstituents were used at a concentration of 10 mg/mL. The biofilms of Candida albicans (ATCC 90028) and Enterococcus faecalis (ATCC 29212) were developed for 7 days and evaluated by metabolic capacity analysis using MTT, cell viability analysis by CFU/mL, and phospholipase activity. The RPMI 1640 medium was used as the negative control and sodium hypochlorite 2.5% and chlorhexidine 2% were used as positive controls. Data were analyzed by a Kruskal–Wallis test and stepwise with adjusted Bonferroni for nonnormal data and an ANOVA one‐way test followed by Tukey’s post hoc test for normal data ( α = 5 % ). Results: The cellular metabolism of the C. albicans and E. faecalis mono‐species biofilms was reduced by cinnamaldehyde and α ‐terpineol ( p < 0.05). For dual‐species biofilm, only α ‐terpineol showed differences compared to the negative control ( p < 0.05). The phytoconstituents showed an inhibitory effect on cell viability (CFU/mL) and phospholipase activity of biofilms, having an activity similar to sodium hypochlorite ( p > 0.05). Conclusions: The phytoconstituents cinnamaldehyde and α ‐terpineol, at a concentration of 10 mg/mL, had an inhibitory effect on mono‐species and dual‐species biofilms of E. faecalis and C. albicans .
DOI: 10.1155/ijm/4769807
Language: English
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,000
OpenAlex ID: W4410340848