Comparison of the characteristics of non-alcoholic fatty liver disease in C57BL/6J-Leprem1hwl/Korl and C57BLKS/J-Leprdb/J mice
Year: 2025
Type: article
Abstract: The Lepr gene encodes a receptor for leptin, a hormone instrumental in the regulation of appetite and metabolism. Mutations in the Lepr gene impair leptin signaling, leading to metabolic dysfunctions and facilitating the development of non-alcoholic fatty liver disease (NAFLD). In this study, we compared the NAFLD-associated phenotypes of two mutant strains of mice, C57BL/6J-Leprem1hwl/Korl (Leprem1hwl) and C57BLKS/J-Leprdb/J (Leprdb/db), carrying different alleles of the Lepr gene. Although both Leprem1hwl and Leprdb/dbmice were characterized by similar obesity phenotypes, leptin resistance, insulin resistance, and glucose intolerance, comparatively, Leprem1hwl mice were found to have relatively more severe hepatic steatosis, along with the upregulated expression of enzymes associated with lipogenesis and triglyceride synthesis, and, notably, the histological characteristics of steatohepatitis were observed only in these mice. In addition, compared with the Leprdb/dbmice, Leprem1hwl mice developed hepatic fibrosis characterized by elevated levels of collagen deposition and expression of profibrotic factors. Moreover, we detected elevated levels of pro-inflammatory mediators and increases in M1 macrophage markers in the serum and liver, respectively, of Leprem1hwl mice. These findings highlight the distinct NAFLD-associated phenotypic differences between Leprem1hwl and Leprdb/dbmice, and thereby indicate that Leprem1hwl mice could serve as a valuable model for studying NAFLD, including steatohepatitis and fibrosis.
Source: EXPERIMENTAL ANIMALS
Authors: Seungwoo Lee, Jae-Hong Min, Myoung Jun Kim, Somi Yun, Min Kyoung Seo, Jong Kwon Lee
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
Funder: Ministry of Food and Drug Safety
Awards: G2441670686, G34649748
OpenAlex ID: W4410353171