Year: 2025
Type: article
Abstract: Coronary atherosclerosis (CAS) is a frequent clinical disease that critically affects human health and requires an early diagnostic marker. MicroRNAs (miRNAs) have the potential to be diagnostic markers due to their aberrant expression in a wide range of diseases. In this study, the levels of miR-411-5p and vasodilator-stimulated phosphoprotein (VASP) were analyzed by qRT-PCR. ROC curves were analyzed for the diagnostic value of miR-411-5p. Human Umbilical Vein Endothelial Cells (HUVECs) were treated with ox-LDL to construct a cell model. Cell proliferation, apoptosis, and inflammatory factors levels were evaluated by the CCK-8, flow cytometry, and Enzyme-linked immunosorbent assay (ELISA). The levels of miR-411-5p were higher in CAS patients than in controls. Ox-LDL promoted the miR-411-5p levels and suppressed VASP in cells. Suppressing miR-411-5p markedly mitigated ox-LDL suppressive effect on cell proliferation while also diminishing its stimulatory roles in apoptosis and inflammation. However, this alleviation was notably reversed when VASP expression was diminished. MiR-411-5p holds promising prospects as a diagnostic marker for CAS, and targeting the miR-411-5p/VASP axis could mitigate the progression of CAS.
Authors: Chang Liu, Yingying Wang, Menghao Sun, Tingqi Shi
Institutions: Nanjing Drum Tower Hospital, Nanjing University
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4410353240